:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies
http://TuEngr.com
ISSN 2228-9860
eISSN 1906-9642
CODEN: ITJEA8
FEATURE PEER-REVIEWED ARTICLE
Vol.13(12)(2022) |
Design of Oligonucleotide Primers for the Development of a Method for Typing Salmonella Isolates in the Russian Federation
Irina Soltynskaya
(The Russian State Center For Animal Feed And Drug Standardization And Quality, Moscow, RUSSIA),
Alexandra Bogomazova (The Russian State Center For Animal Feed And Drug Standardization And Quality, Moscow, RUSSIA),
(Federal Research And Clinical Center Of Physical-Chemical Medicine Federal Medical Biological Agency, Moscow, RUSSIA),
Ekaterina Krylova, Olga Prasolova, Olga Ivanova ( The Russian State Center For Animal Feed And Drug Standardization And Quality, Moscow, RUSSIA).
Discipline: Microbiology, Molecular Biology.
doi: 10.14456/ITJEMAST.2022.251
Keywords:Salmonella Enterica; DNA sequencing; bacterial typing; serovar; CRISPR 1 locus.
AbstractThis article presents the results of work on the creation of molecular genetic methods for the control of industrial and vaccine strains of microorganisms, including methods for typing salmonellosis pathogens to serovar. The technique is based on the sequence analysis of a fragment of a highly polymorphic region of the CRISPR1 locus by Sanger DNA sequencing. Using the reference genomes of Salmonella enterica from databases and our own whole genome sequencing data, we designed oligonucleotide primers and assessed their quality and specificity.Paper ID: 13A12T
Cite this article:
Soltynskaya, I., Bogomazova, A., Krylova, E., Prasolova, O., and Ivanova, O. (2022). Design of Oligonucleotide Primers for the Development of a Method for Typing Salmonella Isolates in the Russian Federation. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 13(12), 13A12T, 1-7. http://TUENGR.COM/V13/13A12T.pdf DOI: 10.14456/ITJEMAST.2022.251
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Other issues:
Vol.13(11)(2022)
Vol.13(10)(2022)
Vol.13(9)(2022)
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