International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

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:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

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ISSN 2228-9860
eISSN 1906-9642
CODEN: ITJEA8


FEATURE PEER-REVIEWED ARTICLE

Vol.13(12)(2022)

  • Design of Oligonucleotide Primers for the Development of a Method for Typing Salmonella Isolates in the Russian Federation

    Irina Soltynskaya (The Russian State Center For Animal Feed And Drug Standardization And Quality, Moscow, RUSSIA),
    Alexandra Bogomazova (The Russian State Center For Animal Feed And Drug Standardization And Quality, Moscow, RUSSIA),
    (Federal Research And Clinical Center Of Physical-Chemical Medicine Federal Medical Biological Agency, Moscow, RUSSIA),
    Ekaterina Krylova, Olga Prasolova, Olga Ivanova ( The Russian State Center For Animal Feed And Drug Standardization And Quality, Moscow, RUSSIA).

    Discipline: Microbiology, Molecular Biology.

    ➤ FullText

    doi: 10.14456/ITJEMAST.2022.251

    Keywords:Salmonella Enterica; DNA sequencing; bacterial typing; serovar; CRISPR 1 locus.

    Abstract
    This article presents the results of work on the creation of molecular genetic methods for the control of industrial and vaccine strains of microorganisms, including methods for typing salmonellosis pathogens to serovar. The technique is based on the sequence analysis of a fragment of a highly polymorphic region of the CRISPR1 locus by Sanger DNA sequencing. Using the reference genomes of Salmonella enterica from databases and our own whole genome sequencing data, we designed oligonucleotide primers and assessed their quality and specificity.

    Paper ID: 13A12T

    Cite this article:

    Soltynskaya, I., Bogomazova, A., Krylova, E., Prasolova, O., and Ivanova, O. (2022). Design of Oligonucleotide Primers for the Development of a Method for Typing Salmonella Isolates in the Russian Federation. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 13(12), 13A12T, 1-7. http://TUENGR.COM/V13/13A12T.pdf DOI: 10.14456/ITJEMAST.2022.251

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Other issues:
Vol.13(11)(2022)
Vol.13(10)(2022)
Vol.13(9)(2022)
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