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:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

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ISSN 2228-9860
eISSN 1906-9642
CODEN: ITJEA8


FEATURE PEER-REVIEWED ARTICLE

Vol.13(5)(2022)

  • LC/MS Profiling of Shilajit Extract for Antimicrobial & Antifungal and Cytotoxic Activities

    Fawzia AlShubaily (Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box 9515, Jeddah, 21589, SAUDI ARABIA),
    Ebtihaj Jambi (Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box 9515, Jeddah, 21589, SAUDI ARABIA and Biomedical Physics Center, King Fahd Medical Research Center, Box 80216, King Abdulaziz University, Jeddah, 21589, SAUDI ARABIA).

    Disciplinary: Biochemistry and Biochemical Engineering.

    ➤ FullText

    DOI: 10.14456/ITJEMAST.2022.98

    Keywords: LC-HRESIMS; Shilajit bioactive compounds; Antimicrobial; Cytotoxicity; LC/MS; Bioactive compounds; Hep G2; Candida albicans; Staphylococcus aureus; DMSO; Microbial strain; Herbal traditional medicine.

    Abstract
    Enormous amounts of bioactive compounds incorporated in the shilajit extracts are accountable for many therapeutic properties. However, little is acknowledged concerning the chemical content and its correlation to the antimicrobial and cytotoxic properties of shilajit extract. Therefore, the current experiment aimed at the profiling of shilajit bioactive compounds with the aid of LC-HRESIMS technology, and assessing the antimicrobial and cytotoxic properties of in vitro and in vivo models. This method allowed the identification of a variety of bioactive compounds, which include fulvic acid, gallic acid, ferulic acid, naphsilajitone, fraxin, 3,8-dihydroxydibenzo-α-pyrone, and pregnane. The results confirmed significant antifungal activity against Staphylococcus aureus at a concentration of 100 μg disc-1, and Candida albicans at concentrations down to 25 μg disc-1 and gave inhibition zones of 13±0.3 and 12±0.3 mm diameter, respectively. There was low inhibition detected at a concentration beneath 25μg disc-1, and null activity of shilajit crude extract in opposition to all the different microbes at the distinct concentrations used in the current study. Cytotoxic percentage inhibition of applied cell lines was elevated via increasing extract concentration and significant percent inhibition (IC50: 19 μg mL-1) of the investigated test extract was revealed by the applied cell line Hep G2. These statistics supply a molecular foundation to explain at least a section of the advisable therapeutic properties of shilajit extract.

    Paper ID: 13A5N

    Cite this article:

    AlShubaily, F. and Jambi, E. (2022). LC/MS Profiling of Shilajit Extract for Antimicrobial & Antifungal and Cytotoxic Activities. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 13(5), 13A5N, 1-13. http://TUENGR.COM/V13/13A5N.pdf DOI: 10.14456/ITJEMAST.2022.98

References

  1. Agarwal SP, et al. Shilajit: a review. Phytotherapy research: PTR. 2007;21(5):401-405.
  2. Carrasco-Gallardo C, Guzman L, and Maccioni RB. Shilajit: a natural phytocomplex with potential procognitive activity. International Journal of Alzheimer's disease. 2012.
  3. Bhattacharya SK. Shilajit attenuates streptozotocin-induced diabetes mellitus and decrease in pancreatic islet superoxide dismutase activity in rats. PTR Phytotherapy Research. 1995;9(1):41-44.
  4. Ghosal S. Chemistry of shilajit, an immunomodulatory Ayurvedic rasayan. Pure and Applied Chemistry. 1990;62(7):1285-1288.
  5. Acharya, SB, et al. Pharmacological actions of Shilajit. Indian journal of experimental biology, 1988. 26(10):775-7.
  6. Jaiswal, AK, and Bhattacharya, S. Effects of Shilajit on memory, anxiety and brain monoamines in rats. Indian J Pharmacol. 1992;24(1):12-7.
  7. Ghosal, S. Shilajit in Perspective, Alpha Science International Limited. 2006, Oxford, United Kingdom.
  8. Wilson, E, et al. Review on shilajit used in traditional Indian medicine. Journal of ethnopharmacology. 2011;136(1):1-9.
  9. Ghosal S, Lal J, and Singh SK. The core structure of Shilajit humus. Soil Biology and Biochemistry, 1991;23(7):673-80.
  10. Schepetkin IA, et al. Complement-fixing activity of fulvic acid from Shilajit and other natural sources. Phytotherapy research: PTR. 2009;23(3):373-84.
  11. Ferraro MJ. Performance standards for antimicrobial disk susceptibility tests. 2000: NCCLS.
  12. Tepe B, et al. Screening of the antioxidative and antimicrobial properties of the essential oils of Pimpinella anisetum and Pimpinella flabellifolia from Turkey. Food Chemistry. 2006;97(4):719-24.
  13. Denizot F, and Lang R. Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of immunological methods. 1986;89(2):271-7.
  14. Dellavalle PD, et al. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chilean J. Agric. Res. Chilean journal of agricultural research. 2011;71(2):231-9.
  15. Naeini A, et al. Anti- Candida albicans activity of some Iranian plants used in traditional medicine. Journal de Mycologie Medicale Journal de Mycologie Medicale. 2009;19(3):168-72.
  16. Srivastava G, et al. Antimicrobial activity of the methanolic extract, fractions and isolated compounds from citrullus colocynthis (l.) schrad. Intl. J. Pharma Bio Sci. International Journal of Pharma and Bio Sciences. 2013;4(3):B825-33.
  17. Bauer R, and Tittel G. Quality assessment of herbal preparations as a precondition of pharmacological and clinical studies. Phytomedicine. International journal of phytotherapy and phytopharmacology, 1996;2(3):193-8.
  18. Springfield EP, Eagles PKF, and Scott G. Quality assessment of South African herbal medicines by means of HPLC fingerprinting. Journal of Ethnopharmacology Journal of Ethnopharmacology. 2005;101(1-3):75-83.
  19. Ravn H, et al., Caffeic acid esters as in vitro inhibitors of plant pathogenic bacteria and fungi. Biochemical Systematics and Ecology Biochemical Systematics and Ecology. 1989;17(3):175-84.
  20. Tawata S, et al. Synthesis and Antifungal Activity of Cinnamic Acid Esters. Bioscience, Biotechnology, and Biochemistry Bioscience, Biotechnology, and Biochemistry. 1996;60(5):909-10.
  21. Fernandez MA, Saenz MT, and Garcia MD. Anti-inflammatory activity in rats and mice of phenolic acids isolated from Scrophularia frutescens. Journal of pharmacy and pharmacology. 1998;50(10):1183.
  22. Kroes BH, et al. Anti-inflammatory activity of gallic acid. Planta medica, 1992. 58(6):499-504.
  23. Mutalik S, et al. Effect of Dianex, a herbal formulation on experimentally induced diabetes mellitus. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2005;19(5):409-15.
  24. Pillai GKG, et al. Antidiabetic potential of polyherbal formulation DB14201: Preclinical development, safety and efficacy studies. Journal of ethnopharmacology. 2017;197:218-30.
  25. Yadav D, et al. In vitro toxicity and antidiabetic activity of a newly developed polyherbal formulation (MAC-ST/001) in streptozotocin-induced diabetic Wistar rats. Protoplasma. 2013;250(3):741-9.
  26. Yuan H, et al. How can synergism of traditional medicines benefit from network pharmacology? Molecules. 2017;22(7):1135.
  27. Chopra R, et al. Indigenous drugs of India, UN Dhur and sons Pvt. Ltd., Calcutta, 1958;289:665.
  28. Binutu OA, Cordell GA. Gallic acid derivatives from Mezoneuron benthamianum leaves. Pharmaceutical Biology. 2000;38(4):284-6.
  29. Mehrotra R. Defense mechanism in plants. Plant pathology. Tata-McGrew Hil, New Delhi, 1997:544p.
  30. Khan NS, Ahmad A, and Hadi S. Anti-oxidant, pro-oxidant properties of tannic acid and its binding to DNA. Chemico-Biological Interactions. 2000. 125(3):177-189.
  31. Windholz, M., et al., The Merck Index. Rahway, NJ: Merck & Co. Inc, 1983. 82:1417-9.
  32. Ghosal, S., et al., Anti-ulcerogenic activity of fulvic acids and 4'-methoxy-6-carbomethoxybiphenyl isolated from shilajit. Phytotherapy Research, 1988. 2(4):187-91.
  33. Schepetkin, I., A. Khlebnikov, and B.S. Kwon, Medical drugs from humus matter: Focus on mumie. DDR Drug Development Research. 2002. 57(3):140-59.
  34. Joone, G.K., J. Dekker, and C.E.J. van Rensburg, Investigation of the Immunostimulatory Properties of Oxihumate. ZEITSCHRIFT FUR NATURFORSCHUNG C. 2003. 58:263-7.
  35. Schepetkin, I.A., et al., Characterization and Biological Activities of Humic Substances from Mumie. J. Agric. Food Chem. Journal of Agricultural and Food Chemistry. 2003. 51(18):5245-54.
  36. Basha, S.A., et al., Effect of Berberine and (+/-)-Bicuculline Isolated from Corydalis chaerophylla on Spore Germination of Some Fungi. FOLIA MICROBIOLOGICA PRAGUE. 2002. 47:161-6.
  37. Kotb-El-Sayed, M.k., H. Amin, and A. Al-kaf, Anti-Microbial, anti-oxidant and anti-ulcerogenic effects of Shilajit on gastric ulcer in rats. Am. J of Biochem Biotech. 2011. 8(1):26-39.
  38. Rachana, S., Antifungal activity screening and HPLC analysis of crude extract from Tectona grandis, Shilajit, Valeriana wallachi. Electronic Journal of Environmental, Agricultural and Food Chemistry. 2009;8(4):218-29.
  39. Hayat, A., Antimicrobial Activity of Shilajit. RADS Journal of Biological Research & Applied Sciences. 2013;4(2):10-2.
  40. Shakirov, D., Experimental treatment of infected wounds with mumie asil'. Eksperimental'naia khirurgiia i anesteziologiia, 1969;14(6):36-9.
  41. Muratova, K. and D. Shakirov, Clinical treatment of suppurative wounds with Mumie. Khirurgiia, 1968;44(9):122-4.
  42. Van Rensburg C, Van Straten A, and Dekker J. An in vitro investigation of the antimicrobial activity of oxifulvic acid. Journal of Antimicrobial Chemotherapy. 2000; 46(5):853-4.
  43. Galimov E, et al., Geochemistry of mummiyo. Geochemistry. 1986;1494-505.
  44. Leighann S, et al. Investigating the biological properties of carbohydrate derived fulvic acid (CHD-FA) as a potential novel therapy for the management of oral biofilm infections. BMC Oral Health. 2013;13(1):47.
  45. Phaechamud T, et al. Some Biological Activities and Safety of Mineral Pitch. Science, Engineering and Health Studies. Silpakorn University Science And Technology Journal. 2008;2(2):7-17.


Other issues:
Vol.13(4)(2022)
Vol.13(3)(2022)
Vol.13(2)(2022)
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