International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies


:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

ISSN 2228-9860
eISSN 1906-9642



  • LC/MS Profiling of Shilajit Extract for Antimicrobial & Antifungal and Cytotoxic Activities

    Fawzia AlShubaily (Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box 9515, Jeddah, 21589, SAUDI ARABIA),
    Ebtihaj Jambi (Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box 9515, Jeddah, 21589, SAUDI ARABIA and Biomedical Physics Center, King Fahd Medical Research Center, Box 80216, King Abdulaziz University, Jeddah, 21589, SAUDI ARABIA).

    Disciplinary: Biochemistry and Biochemical Engineering.

    ➤ FullText

    DOI: 10.14456/ITJEMAST.2022.98

    Keywords: LC-HRESIMS; Shilajit bioactive compounds; Antimicrobial; Cytotoxicity; LC/MS; Bioactive compounds; Hep G2; Candida albicans; Staphylococcus aureus; DMSO; Microbial strain; Herbal traditional medicine.

    Enormous amounts of bioactive compounds incorporated in the shilajit extracts are accountable for many therapeutic properties. However, little is acknowledged concerning the chemical content and its correlation to the antimicrobial and cytotoxic properties of shilajit extract. Therefore, the current experiment aimed at the profiling of shilajit bioactive compounds with the aid of LC-HRESIMS technology, and assessing the antimicrobial and cytotoxic properties of in vitro and in vivo models. This method allowed the identification of a variety of bioactive compounds, which include fulvic acid, gallic acid, ferulic acid, naphsilajitone, fraxin, 3,8-dihydroxydibenzo-α-pyrone, and pregnane. The results confirmed significant antifungal activity against Staphylococcus aureus at a concentration of 100 μg disc-1, and Candida albicans at concentrations down to 25 μg disc-1 and gave inhibition zones of 13±0.3 and 12±0.3 mm diameter, respectively. There was low inhibition detected at a concentration beneath 25μg disc-1, and null activity of shilajit crude extract in opposition to all the different microbes at the distinct concentrations used in the current study. Cytotoxic percentage inhibition of applied cell lines was elevated via increasing extract concentration and significant percent inhibition (IC50: 19 μg mL-1) of the investigated test extract was revealed by the applied cell line Hep G2. These statistics supply a molecular foundation to explain at least a section of the advisable therapeutic properties of shilajit extract.

    Paper ID: 13A5N

    Cite this article:

    AlShubaily, F. and Jambi, E. (2022). LC/MS Profiling of Shilajit Extract for Antimicrobial & Antifungal and Cytotoxic Activities. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 13(5), 13A5N, 1-13. http://TUENGR.COM/V13/13A5N.pdf DOI: 10.14456/ITJEMAST.2022.98


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