International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies


:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

ISSN 2228-9860
eISSN 1906-9642



  • Comparison of the Microbiota of the Reproductive Tract and the Mammary Gland of Cows with and Without Inflammation Using 16S rRNA Sequencing

    O.V. Sokolova, N.A. Bezborodova, M.V. Bytov , V.D. Zubareva, I.A. Shkuratova, O.S. Zaitseva, N.A. Martynov (FSBSI (Ural Federal Agrarian Scientific Research Centre, UrB of RAS), Ekaterinburg, RUSSIA).

    Disciplinary: Microbiology, BioScience.

    ➤ FullText

    DOI: 10.14456/ITJEMAST.2022.108

    Keywords: 16S rRNA, metagenomics, Holstein cattle, microbiota, mammary gland, reproductive tract.

    Inflammatory diseases of the mammary gland and reproductive tract of cattle cause the greatest economic damage to dairy farming. Analysis of the relationship between the microbiota of these loci in animals without pathology and with mastitis and endometritis will provide new knowledge about the etiology of inflammatory diseases. This study performed sequencing of 16S rRNA variable regions, to determine and compare the composition of the mammary gland and the reproductive tract microbiota of cattle in normal conditions and inflammation. This research comprised 81 animals from five agricultural organizations of the Sverdlovsk oblast (Ural region, Russia), located in different areas. In each, 4 groups of experimental animals were formed: Group 1 animals with no pathology, i.e. without signs of inflammation of the mammary gland and reproductive tract (NP); Group 2 animals with signs of the mammary gland inflammation, but without signs of the reproductive tract inflammation (M); Group 3 animals with signs of the reproductive tract inflammation, but without signs of the mammary gland inflammation (E); Group 4 animals with signs of inflammation of the mammary gland and reproductive tract (EM). Samples of biological material (mammary gland secretion, cervical swabs) were obtained from each cow of all experimental groups; for further studies, the method of pooled sample for 16S rRNA metagenomic analysis was used. A comparison was made of the microbiota of the mammary gland and the reproductive tract of cattle with and without inflammatory processes.

    Paper ID: 13A6C

    Cite this article:

    Sokolova, O.V., Bezborodova, N.A., Bytov, M.V., Zubareva, V.D., Shkuratova, I.A., Zaitseva, O.S., Martynov, N.A.. (2022). Comparison of the Microbiota of the Reproductive Tract and the Mammary Gland of Cows with and Without Inflammation Using 16S rRNA Sequencing. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 13(6), 13A6C, 1-12. http://TUENGR.COM/V13/13A6C.pdf DOI: 10.14456/ITJEMAST.2022.108


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