An Application of Loop Isothermal Amplification to Detect African Swine Fever Virus

Gnezdilova, Larisa; Pozyabin, Sergey; Borunova, Seidfatima; Stoyan, Tatyana; Selina, Marina; Davydova, Ekaterina

:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

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ISSN 2228-9860
eISSN 1906-9642
CODEN: ITJEA8

FEATURE PEER-REVIEWED ARTICLE

Vol.13(9)(2022)

An Application of Loop Isothermal Amplification to Detect African Swine Fever Virus

Larisa Gnezdilova (Department of Disease Diagnostics, Therapy, Obstetrics and Animal Reproduction, Moscow State Academy of Veterinary Medicine and Biotechnology - MBA named after K.I. Scriabin, Moscow, RUSSIA),
Sergey Pozyabin ( Department of Veterinary Surgery, Moscow State Academy of Veterinary Medicine and Biotechnology - MBA named after K.I. Scriabin, Moscow, RUSSIA),
Seidfatima Borunova (Basic Department of Biological Safety of Veterinary Surveillance Objects and Drug Circulation in Veterinary Medicine, Moscow State Academy of Veterinary Medicine and Biotechnology - MBA named after K.I. Scriabin, Moscow, RUSSIA),
Tatyana Stoyan ( LLC OD-TEST, Moscow, RUSSIA),
Marina Selina (Department of Information Technology, Mathematics and Physics, Moscow State Academy of Veterinary Medicine and Biotechnology - MBA named after K.I. Scriabin, Moscow, RUSSIA),
Ekaterina Davydova ( LLC , Moscow, RUSSIA).

Discipline: Veterinary.
➤ FullText

doi: 10.14456/ITJEMAST.2022.177

Keywords: Loop-mediated isothermal amplification, LAMP, Virus ASF, PCR diagnostics

Abstract
Loop isothermal amplification (LAMP) is an alternative to PCR that reduces the time of analysis and does not require the use of expensive equipment. Here, we describe our real-time LAMP technique with hybridization fluorescence detection for the detection of ASF virus in biological material from animals. The detection limit of the technique is 2*103 copies/ml, which is comparable to the sensitivity of PCR. The high specificity of the method developed by LAMP was shown, and no false positive results were observed when testing other viruses and microorganisms, as well as biological samples that did not contain DNA of the ASF virus. In comparative tests on blood plasma samples (n=33) and a suspension of pathological material of the tonsils, spleen, lungs (n=30), the results of PCR and LAMP coincided almost completely, with the exception of one LAMP false-negative sample with a low viral load (PCR Ct ~ 38).
Paper ID: 13A9I
Cite this article:

Gnezdilova, L., Pozyabin, S., Borunova, S., Stoyan, T., Selina, M., and Davydova, E. (2022). An Application of Loop Isothermal Amplification to Detect African Swine Fever Virus. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 13(9), 13A9I, 1-9. http://TUENGR.COM/V13/13A9I.pdf DOI: 10.14456/ITJEMAST.2022.177

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