Andrei Koshchayev (Department of Biotechnology, Biochemistry and Biophysics, Kuban State Agrarian University named after I.T. Trubilin, RUSSIA),
Albina Luneva (Department of Parasitology, Veterinary Examination and Pet Hygiene, Kuban State Agrarian University named after I.T. Trubilin, RUSSIA),
Yury Lysenko (Department of Biotechnology, Biochemistry and Biophysics, Kuban State Agrarian University named after I.T. Trubilin, RUSSIA),
Alexander Lysenko, and Azamat Shantyz (Department of Therapy and Pharmacology, Kuban State Agrarian University named after I.T. Trubilin, RUSSIA).
Disciplinary: Biotechnology, Bioscience.
Keywords: Acute toxicity, Chronic toxicity, Lactobacillus, Harmlessness, Toxigenicity, Probiotic, Virulence, Probiont strains, Mice, Rats.
The research work presents the results of studying the virulence, toxigenicity, and various types of toxicity of probiont strains isolated and identified by modern molecular genetic and microbiological methods from wild birds' gastrointestinal tract. In this work, laboratory animals were used for research, particularly nonlinear mice, rats, and albino rabbits. As a result of a complex of preclinical studies, it was revealed that experimental autochthonous strains of lactobacilli do not show signs of virulence, toxicity, toxicity, and a positive reaction to the production of hemolysin. In experiments on acute and chronic toxicity, regardless of the concentration of the studied strains introduced into the organism of laboratory animals, as well as their joint consortium in the composition of the probiotic, no death of the experimental animals was revealed, there were no signs of health disorders and body weight loss during the experiment. In general, the study results showed that the studied cultures of microorganisms and their consortium do not have a toxic effect on the organism of laboratory animals, both with a single and long-term use in the diet, which confirms their safety.
Paper ID: 12A1L
Cite this article:
Nawaz, R., Bakhsh, K. Sami Ullah, Rehman, S., Azeem, M., Hayat, A. (2021). Biosafety of Autochton Strains of Microorganisms-Probion and Their Joint Composition. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 12(1), 12A1L, 1-11. http://doi.org/10.14456/ITJEMAST.2021.12
Lysenko Yu.A. (2020). Development and pharmacological substantiation of the use of new feed additives of microbial origin in poultry meat farming. Dissertation of the doctor of biological sciences, 476p.
Guidelines for the sanitary and epidemiological assessment of the safety and functional potential of probiotic microorganisms used for food production (MU 188.8.131.5289-10), 104p.
System for pre-registration preclinical study of drug safety. Selection, verification and storage of industrial strains used in the production of probiotics. Methodical instructions (MUK 4.2.2602-10), 60p.
Sanitary and Epidemiological Rules. Sanitary and Epidemiological Requirements for the Design, Equipment and Maintenance of Experimental Biological Clinics (Vivariums). SP 184.108.40.20618-14, 7p.
Radchenko V.V., Ilnitskaya E.V., Shuvaeva T.M., Alexandrova M.V., Luneva A.V., Lysenko Yu.A., Basova N.Yu., Nekrasov A.N., Koschaev A.G. (2020). Comparative analysis and probiotic potential of new strains of the genus Lactobacillus from evolutionarily fixed associations of the gastrointestinal tract of wild birds. Biopharmaceutical Journal, 12(1), 44-49.
Lysenko Yu.A., Koshchaev A.G., Lysenko A.A., Omarov R.S., Shlykov S.N. (2020). Biological properties of microorganisms isolated from drone milk of honeybees. International Transaction Journal of Engineering, Management and Applied Sciences and Technologies, 11(12), 11A12K. DOI: 10.14456/ITJEMAST.2020.236
Luneva A., Lysenko Y., Koshchaev A., Nesterenko A., Guzenko V. (2019). Comprehensive biosafety assessment of additives based on live microorganisms. International Journal of Engineering and Advanced Technology, 9(1), 2477-2483.
Luneva A, Koshchayev A, Nesterenko A, Volobueva E, Boyko A. (2020). Probiotic potential of microorganisms obtained from the intestines of wild birds. International Transaction Journal of Engineering, Management and Applied Sciences and Technologies, 11(12), 11A12E. DOI: 10.14456/ITJEMAST.2020.230
Call-for-Research Papers: ITJEMAST invites you to submit high quality papers for full peer-review and possible publication in areas pertaining engineering, science, management and technology, especially interdisciplinary/cross-disciplinary/multidisciplinary subjects.
To publish your work in the next available issue, your manuscripts together with copyright transfer document signed by all authors can be submitted via email to Editor @ TuEngr.com (no space between). (please see all detail from Instructions for Authors
Publication and peer-reviewed process:
After the peer-review process (4-10 weeks), articles will be on-line published in the available next issue. However, the International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies
cannot guarantee the exact publication time as the process may take longer time, subject to peer-review approval and adjustment of the submitted articles.