International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies


:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

ISSN 2228-9860
eISSN 1906-9642


Vol.11(14) (2020)
(Special Issue: Selected Articles from IDSISA2020 Conference @ Russia)


    N.P. Buryakov( Department of the Feeding Animals, Faculty Animal Science and Biology, FSBEI HE, Russian State Agrarian University, Moscow Timiryazev Agricultural Academy, Moscow, RUSSIA),
    Laptev G.Yu., Ilina L.A. (Plemzavod Maisky Agricultural Production Cooperative, Vologda Region, RUSSIA ),
    Buryakova M.A., Zaikina A.S. ( Department of the Feeding Animals, Faculty Animal Science and Biology, FSBEI HE, Russian State Agrarian University, Moscow Timiryazev Agricultural Academy, Moscow, RUSSIA),
    Kasatkina I.A. (Biotrof Company, Saint-Petersburg, RUSSIA),
    D.E. Aleshin (Department of the Feeding Animals, Faculty Animal Science and Biology, FSBEI HE, Russian State Agrarian University, Moscow Timiryazev Agricultural Academy, Moscow, RUSSIA).

    Disciplinary: Farm Animals, Cattle Feeding Technology, Agricultural Biology, Livestock and Dairy Science, Veterinary Science, Microbiology, Biotechnology, Biochemistry, Livestock Products Technology.

    ➤ FullText

    DOI: 10.14456/ITJEMAST.2020.268

    Keywords: Animal husbandry; Lactating cows; Protein concentrate (PC); Cattle feeding; Feed of animal origin; Feed nutrition; Blood biochemistry; Ruminal microbiome; Symbiotic microflora; Non-separable protein (NSP).

    The wholesome feeding of animals requires a constant supply of new knowledge about the processes of digestion, use, and transformation of nutrients from the diet, as well as about the processes of animal body protein synthesis, which would give an incentive to adjust existing feeding standards. This article contains experimental data obtained on lactating highly productive cows with milk productivity of more than 8000 kg per year with the use of protein concentrate (PC) as part of the diet. PC "Agro-Matic" is a product that includes components of plant origin (seeds of Lupinus albus L.) and production waste of poultry meat. The studied results of PC Agro-Matic present in comparison with other protein feeds, the biochemical status data of the body, and the composition of the ruminal bacterial community of the cows while including PC as part of diets for cows in the amount of 1.0 kg and 1.5 kg, respectively. Analysis of the biochemical parameters data of the blood of cows fed different levels of PC showed that all the studied indicators values were within the physiological norm. In all experimental groups of animals, there were found no significant deviations in the number of pathogenic microorganisms in the rumen fluid of lactating cows in one-third of lactation.

    Paper ID: 11A14A

    Cite this article:

    Buryakov, N.P., Laptev, G.Yu., Ilina, L.A., Buryakova, M.A., Zaikina, A.S., Kasatkina, I.A., Aleshin, D.E. (2020). USING PROTECTED PROTEIN SOURCE SUPPLEMENTATION ON MICROORGANISMS OF RUMEN AND BIOCHEMICAL STATUS LACTATING COWS. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 11(14), 11A14A, 1-14.


    Afanasieva, A.I. et al. (2007). The influence of the structure of the feeding diet on the morphobiochemical parameters of blood and the level of milk productivity of red steppe cows. Bulletin of the Altai state agrarian University, 9(35), 36-40.

    Alexandrov, Yu.A. (2015). Dynamics of biochemical indicators of blood of cows with different levels of milk productivity. Bulletin of the Mari State University, 3, 5-9.

    Puniya, A.K., et al. (2015). Rumen Microbiology: From Evolution to Revolution Springer, New Deli, 379.

    Antonova V.S., et al. (2011). Methodology of scientific research in animal husbandry. Orenburg, 246.

    Bach, A., et al. (2005). Nitrogen metabolism in the rumen. Journal of Dairy Science, 88, E9-E21.

    Barchiesi-Ferrari, C., & R. Anrique (2011). Ruminal Degradability of Dry Matter and Crude Protein from Moist Dehulled Lupin and Extruded Rapeseed Meal. Chilean Journal of Agricultural Research, 71(3), 430-436.

    Berry D.P., et al. (2003). Genetic relationships among body condition score, body weight, milk yield, and fertility in dairy cows. Journal of Dairy Science. 86(6): 2193-2204. DOI: 10.3168/jds.s0022-0302(03)73809-0

    Buryakov N.P, et al. (2019). Efficiency of white lupin grain in composition of feed for dairy cattle. 18th International Scientific Conference Engineering for Rural Development. Jelgava, 407-412. DOI: 10.22616/ERDev2019.18.N237

    Buryakov, N.P. & A.E. Stavtcev. (2019). Influence of PC in the diet on productivity and amino acid composition of cow milk. IOP Conference Series: Earth and Environmental Science, 341(1), 012057

    Cherdthong A., et al. (2014). Effects of replacing soybean meal with dried rumen digesta on feed intake, digestibility of nutrients, rumen fermentation and nitrogen use efficiency in Thai cattle fed on rice straw. Livestock Science. 169: 71-77.

    Cunha, T.J. (2010). Nitrogen and Energy Nutrition of Ruminants. Elsevier, 358.

    Dushkin, E.V. et al. (2011). Features of changes in the NEFA in the blood and milk of cows depending on the different composition of concentrated feed in the diet. Niva Of The Volga Region, 2(19), 90-94.

    Fedorenko, V.F., et al. (2017). Deep processing of agricultural raw materials. Rosinformagrotech, 160.

    Fisinin, V.I., et al. (2017). Deep processing of secondary products of the poultry industry for different areas of use. Agricultural biology, 52(6), 1105-1115.

    Flint H.J. & Thomson, A.M. (1990). Deoxyribonuclease activity in isolated rumen bacteria and rumen fluid. Lett. Appl. Microbiol, 11, 18-21.

    Geishauser, T., et al. (2012). Factors associated with ruminal pH at herd level. Journal of Dairy Science, 95(8), 4556-4567.

    Golovin, A. G., et al., (2016), Recommendations for detailed feeding of dairy cattle: reference guide. L.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy, 242.

    Gonzlez F.D., et al. (2011). Relationship among blood indicators of lipomobilization and hepatic function during early lactation in high-yielding dairy cows. Journal of Veterinary Science, 12(3), 251-255.

    Gonzlez J., et al. (1998). Rumen degradability and microbial contamination of fish meal and meat meal measured by the in situ technique. Animal Feed Science and Technology, 73(1-2), 71-84.

    Grushkin, A.G., Shevelev, N.S. (2008). On the morphofunctional features of the ruminant rumen microbiota and the role of cellulolytic bacteria in rumen digestion. Agricultural biology, 2, 12-19.

    Hall M.B. & Huntington G.B. (2008). Nutrient synchrony: Sound in theory, elusive in practice1. Journal of Animal Science. 86(14): E287-E292.

    Henderson G., et al. (2019). Improved taxonomic assignment of rumen bacterial 16S rRNA sequences using a revised SILVA taxonomic framework. Peer J., 7.

    Holman, D. B., & K.E. Gzyl. (2019). A meta-analysis of the bovine gastrointestinal tract microbiota. FEMS Microbiology Ecology. DOI:10.1093/femsec/fiz072

    Ipharraguerre I.R. & Clark J.H. (2005). Impacts of the Source and Amount of Crude Protein on the Intestinal Supply of Nitrogen Fractions and Performance of Dairy Cows. Journal of Dairy Science. 88: E22-E37.

    Juho, K. (2010). Lypsylehm?n ruokinta Painos: 1. Pianos, ProAgria Keskusten Liitto, 140.

    Laptev G.Yu., et al. (2016). Regulations on the content of microflora in the rumen of cattle. Saint-Petersbur, 46.

    Leibova, V.B.. et al. (2011). Metabolic state at the end of the milking period in highly productive black-and-white cows. Agricultural biology, 6, 103-109.

    Masucci F., et al. (2006). Effect of Lupinus albus as protein supplement on yield, constituents, clotting properties and fatty acid composition in ewes' milk. Small Ruminant Research, 65(3), 251-259.

    McDonald P. (2001). Animal Nutrition. 7th Ed. Edited by P. McDonald, et al., Washington, 408.

    National Ressearch Council. (2001). Nutrient requirements of dairy cattle. 7th Ed. National Academy Press, Washington, USA. Overton T.R. & M.R. Waldron. (2004). Nutritional Management of Transition Dairy Cows: Strategies to Optimize Metabolic Health. Journal of Dairy Science. 87: E105-E119.

    Pethick D.W., et al. (1987). Non-esterified long chain fatty acids metabolism in feed sheep at rest and during exercise. Australian Journal of Biological Science, 40(2). 221-234.

    Piva G., et al. (2001). Effect of temperature, pressure and alkaline treatments on meat meal quality. Animal Feed Science and Technology, 89(1-2), 59-68.

    Pivnyak, I.G. & B.V. Tarakanov. Microbiology of ruminant digestion. ?oscow: Colos, 1982, 247.

    Poghosyan, D.G. (2014). The quality of protein in feeds for ruminant animals: a monograph. Penza: RIO PGSKHA, 133.

    Rode, L.M. (2000). Maintaining a healthy rumen review. Advances in Dairy Technology. 12,101-108.

    Romanenko, L.V., V.I. Volgin. (2007). Features of feeding and ration systems for highly productive dairy cows. Agricultural biology, 4, 20-28.

    Ryadchikov, V.G. (2006) Norms and rations of feeding of agricultural animals: methodology, errors, prospects. Agricultural biology. 4: 68-81.

    Samburov, N.V., et al. (2012). Assessment of the state of metabolism in highly productive cows. Bulletin of the Kurgan agricultural Academy, 1, 83-86.

    Schingoethe, D.J. (1996). Dietary influence on protein level in milk and milk yield in dairy cows. Animal Feed Science and Technology, 60(3-4), 181-190.

    Silva A.L., et al. (2018). Effects of rumen undegradable protein on intake, digestibility and rumen kinetics and fermentation characteristics of dairy heifers. Animal Feed Science and Technology. 244, 1-10.

    Sizova, Yu.V. & A.S. Zelenina. (2010). Productivity and technological properties of cow's milk with different amino acid composition of the exchange protein in the diet. Agricultural biology, 6, 50-53.

    Slanina, L., et al. (1992). The metabolic profile of cattle in relation to health and production (in Slovak). Ustav vedeckych informacii a osvety, Bratislava, 115.

    Soriani N., et al. (2012). Relationships between rumination time, metabolic conditions, and health status in dairy cows during the transition period. Journal of Animal Science. 90(12), 4544-4554.

    Svarich, D.A., Truchachev V.I., & N.Z. Zladnev. (2007). Productivity of cows with different protein breakdown in the rumen. Problems of biology of productive animals, 2, 109-119.

    Terramoccia S., et al. (2000). Protein and protein-free dry matter rumen degradability in buffalo, cattle and sheep fed diets with different forage to concentrate ratios. Livestock Production Science. 65(1-2), 185-195.

    Hackman, T.J., Firkins, J.L. (2015). Maximizing efficiency of rumen microbial protein production. Frontiers in Microbiology. doi: 10.3389/fmicb.2015.00465

    Trafimov, I.A. (2001). Metabolism and productivity. Metabolic disorders in highly productive cows under various conditions of keeping and feeding. Agricultural biology, 2, 27-41.

    Vashchekin, E.P. (2005). Metabolism of nitrogenous substances in repair bulls with different sources of feed protein in the diet. Agricultural biology, 6, 40-45.

    Volgin, V.I., & E.A. Korochkina. (2018). Full-fledged feeding of dairy cattle is the basis for realizing the genetic potential of productivity, Moscow. RAS, 260.

    Wattie, M.A, V.T. Howard (2003). Basic aspects of milk production, Madison: U.S. Livestock Genetics Export, 139.

    Weimer P.J. (2015). Redundancy, resilience, and host specificity of the ruminal microbiota: implications for engineering improved ruminal fermentations. Frontiers in Microbiology, 6, 296-312.

    Yokoyama M.T., K.A. Johnson. (1988). Microbiology of the rumen and intestine, in the ruminant animal digestive physiology and nutrition. D.C. Church, ed. Wavel and Press, Inc., IL, 145-171

    Yu P., et al. (2002). An analysis of the nutritive value of heat processed legume seeds for animal production using the DVE/OEB model: a review. Animal Feed Science and Technology, 99(1-4), 141-176.

    Yudin, M.F. (2001). Diseases. The physiological state of the body of cows in different seasons of the year. Veterinary science, 2, 38-56.

Other issues:


Call-for-Scientific Papers
Call-for-Research Papers:
ITJEMAST invites you to submit high quality papers for full peer-review and possible publication in areas pertaining engineering, science, management and technology, especially interdisciplinary/cross-disciplinary/multidisciplinary subjects.

To publish your work in the next available issue, your manuscripts together with copyright transfer document signed by all authors can be submitted via email to Editor @ (no space between). (please see all detail from Instructions for Authors)

Publication and peer-reviewed process:
After the peer-review process (4-10 weeks), articles will be on-line published in the available next issue. However, the International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies cannot guarantee the exact publication time as the process may take longer time, subject to peer-review approval and adjustment of the submitted articles.