:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies
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ISSN 2228-9860
eISSN 1906-9642
CODEN: ITJEA8
FEATURE PEER-REVIEWED ARTICLE
Vol.12(4) (2021) |
Biotechnology of Cultivation of Probiotic Lactobacilli
Yury Lysenko (Department of Microbiology, Epizootology and Virology, Kuban State Agrarian University named after I.T. Trubilin, RUSSIA),
Nadezhda Machneva (Department of Biotechnology, Biochemistry and Biophysics, Kuban State Agrarian University named after I.T. Trubilin, RUSSIA),
Anatoly Smirnov (Laboratory of Veterinary Sanitation and Ecological Safety in Beekeeping, All-Russian Scientific Research Institute of Veterinary Sanitation, Hygiene and Ecology, RUSSIA),
Alexander Panin (Department of Immunology and Biotechnology, Moscow State Academy of Veterinary Medicine and Biotechnology - MVA by K.I. Skryabin, RUSSIA),
Andrey Koshchaev (Department of Biotechnology, Biochemistry and Biophysics, Kuban State Agrarian University named after I.T. Trubilin, RUSSIA).
Disciplinary: Microbiology, Agricultural Biotechnology.
doi: 10.14456/ITJEMAST.2021.72
Keywords: Lactobacilli cultivation; Nutrient medium; Growth requirements; Reducing sugars; Microorganism's growth.
AbstractThis article presents the research results on the selection of optimal conditions for the cultivation of probiotic microorganisms isolated from honey bee drone milk for increasing the biomass of the studied lactobacilli. To determine microorganisms' growth requirements, we used the developed molasses medium and conventional nutrient media for lactobacilli. As a result of calculating the number of lactic acid bacteria on agar nutrient medium, it was found that molasses medium has optimal growth requirements, which includes 45.0 g/l of fodder molasses (50% beet molasses and 50% corn molasses), K2HPO4 2.0 g/l, yeast extract 0.02 g/l. Simultaneously, the developed medium promoted the increase in the titer of microorganisms up to 5.0 x 1010 CFU/ml. The amount of the studied Lactobacillus kunkeei reached 2.3-4.7 x 109 CFU/ml on standard nutrient media. When studying the effect of different temperatures and growing times on lactic acid bacteria's biomass growth, the optimal cultivation conditions are 37°C and 24 h. It was proved that the maximum depletion of the nutrient medium's carbon component occurs 24-28 hr from the start of the microorganism growth process.Paper ID: 12A4I
Cite this article:
Lysenko, Y., Machneva, N., Smirnov, A., Panin, A., Koshchaev, A. (2021). Biotechnology of Cultivation of Probiotic Lactobacilli. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 12(4), 12A4I, 1-10. http://doi.org/10.14456/ITJEMAST.2021.65
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