International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies


:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

ISSN 2228-9860
eISSN 1906-9642


Vol.11(15) (2020)

  • Diagnoses of Leptospirosis in Animals

    Gulnara Nurlygayanova, Vasily Belousov, Alisa Varentsova (Department of Short Research Papers, Federal State Budgetary Institution, Central Scientific and Methodical Veterinary Laboratory, RUSSIA),
    Nadezhda Ozheredova (Department of Epizootology and Microbiology, Stavropol State Agrarian University, RUSSIA),
    Oleg Chernykh (Department of Microbiology, Epizootology and Virology, Kuban State Agrarian University named after I. T. Trubilina, RUSSIA).

    Disciplinary: Biology, Epizootology, and Microbiology, Bioscience, Veterinary Sciences.

    ➤ FullText

    DOI: 10.14456/ITJEMAST.2020.304

    Keywords: Dark-Field Microscopy; Microagglutination Reaction; PCR; Bacteriological studies; Microagglutination Reaction; Infectious jaundice; Microscopic agglutination test (MAT).

    There have been presenting a comparative analysis of the results of leptospirosis diagnosis by methods used in the Russian Federation's veterinary laboratories. Using the microagglutination reaction, specific antibodies to leptospira were detecting in 136862 animals. A total of 64686 urine samples was examined with dark-field microscopy. Applying PCR, the leptospira genome is detected in pathological material in 57 cases. The PCR method in the water of open reservoirs cannot detect the pathogenic leptospira genome. In the study of biological material, the leptospira genome was found in 248 cases.

    To increase the efficiency of diagnosis and reduce the time of diagnosis for leptospirosis, it is advisable to conduct parallel bacteriological studies of the material in combination with PCR analysis. Micro-agglutination reaction and molecular genetic analysis (PCR) are the most inforeaction micro-agglutinative and technologically advanced laboratory diagnostic methods.

    Paper ID: 11A15P

    Cite this article:

    Nurlygayanova, G., Belousov, V., Varentsova, A., Ozheredova, N., Chernykh, O. (2020). Diagnoses of Leptospirosis in Animals. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 11(15), 11A15P, 1-9.


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