International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

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:: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies

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ISSN 2228-9860
eISSN 1906-9642
CODEN: ITJEA8


FEATURE PEER-REVIEWED ARTICLE

Vol.11(15) (2020)

  • Diagnoses of Leptospirosis in Animals

    Gulnara Nurlygayanova, Vasily Belousov, Alisa Varentsova (Department of Short Research Papers, Federal State Budgetary Institution, Central Scientific and Methodical Veterinary Laboratory, RUSSIA),
    Nadezhda Ozheredova (Department of Epizootology and Microbiology, Stavropol State Agrarian University, RUSSIA),
    Oleg Chernykh (Department of Microbiology, Epizootology and Virology, Kuban State Agrarian University named after I. T. Trubilina, RUSSIA).

    Disciplinary: Biology, Epizootology, and Microbiology, Bioscience, Veterinary Sciences.

    ➤ FullText

    DOI: 10.14456/ITJEMAST.2020.304

    Keywords: Dark-Field Microscopy; Microagglutination Reaction; PCR; Bacteriological studies; Microagglutination Reaction; Infectious jaundice; Microscopic agglutination test (MAT).

    Abstract
    There have been presenting a comparative analysis of the results of leptospirosis diagnosis by methods used in the Russian Federation's veterinary laboratories. Using the microagglutination reaction, specific antibodies to leptospira were detecting in 136862 animals. A total of 64686 urine samples was examined with dark-field microscopy. Applying PCR, the leptospira genome is detected in pathological material in 57 cases. The PCR method in the water of open reservoirs cannot detect the pathogenic leptospira genome. In the study of biological material, the leptospira genome was found in 248 cases.

    To increase the efficiency of diagnosis and reduce the time of diagnosis for leptospirosis, it is advisable to conduct parallel bacteriological studies of the material in combination with PCR analysis. Micro-agglutination reaction and molecular genetic analysis (PCR) are the most inforeaction micro-agglutinative and technologically advanced laboratory diagnostic methods.

    Paper ID: 11A15P

    Cite this article:

    Nurlygayanova, G., Belousov, V., Varentsova, A., Ozheredova, N., Chernykh, O. (2020). Diagnoses of Leptospirosis in Animals. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 11(15), 11A15P, 1-9. http://doi.org/10.14456/ITJEMAST.2020.304



References:

Ananyina, Yu.V. (2010). Leptospirosis of humans and animals: distribution trends and prevention problems. Epidemiology and Vaccine Prevention, 2(51), 13-16.

Bal, A.E., Gravekamp, C., Hartskeerl, T.R.A., De Meza-Brewster, J., Korver, H., Terpstra, W.J. (1994). Detection of Leptospires in Urine by PCR for Early Diagnosis of Leptospirosis. Journal of Clinical Microbiology, 1894-1898.

Belousov, V.I., Nurlygayanova, G.A., Varentsova, A.A., Bazarbayev, S.B. (2018). Laboratory diagnosis and improvement of the prevention of leptospirosis in animals in the Russian Federation. Proceedings of the All-Russian Research Institute of Experimental Veterinary Medicine named after Ya.R. Kovalenko, 80(2), 46-52.

Bolotsky I.A., Sementsov, V.I., Prutsakov, S.V., Vasiliev, A.K., Pakhomov, A.V. (2009). The epizootic role of wild animals in leptospirosis in the North Caucasus. Journal of Veterinary Medicine of the Kuban, 3, 15-16.

Budihal, S.V., Perwez, K. (2014). Leptospirosis Diagnosis: Competency of Various Laboratory Tests. Journal of Clinical and Diagnostic Research, 8(1), 199-202.

Losí-Yatsenko N.G., Karimov, I.Z., Pavlenko, A.L., Kozlovsky, O.A. (2011). Leptospirosis in the Crimea - a problem not only of infectious diseases. Krimsky therapeutic journal, 1, 83-88.

Malakhov, Yu.A., Panin, A.N., Soboleva, G.L. (2000). Leptospirosis of animals. Yaroslavl. DIA-press, 584.

Soboleva, G.L., Ananyina, Yu.V., Nepoklonova, I.V. (2017). Actual issues of leptospirosis in humans and animals. Russian Veterinary Journal, 8, 13-17.

Stoyanova, N.A., Tokarevich, N.K., Vaganova, A.N., Gracheva, L.I., Mayorova, S.O. (2010). Leptospirosis: a manual for doctors. Edited by Yu.V. Ananyina, SPb. NIIEM them. Pasteur, 116.

World Health Organization (2003). Human leptospirosis: guidance for diagnosis, surveillance, and control. 109p.



Other issues:
Vol.11(14)(2020)
Vol.11(13)(2020)
Vol.11(12)(2020)
Vol.11(11)(2020)
Vol.11(10)(2020)
Vol.11(9)(2020)
Vol.11(8)(2020)
Vol.11(7)(2020)
Vol.11(6)(2020)
Vol.11(5)(2020)
Vol.11(4)(2020)
Vol.11(3)(2020)
Vol.11(2)(2020)
Vol.11(1)(2020)
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